12th European Nitrogen Fixation Conference
 12th European Nitrogen Fixation Conference

Scientific program

Overview timetable

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Book of abstracts


Detailed program



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25th August (Thursday)






Introduction to the Kondorosi Award


Presentation of the Kondorosi Awardee


Lecture of the Kondorosi Awardee


EMBO Lecture
Jens Stougaard
Gatekeepers of symbiotic nitrogen fixation in legumes




26th August (Friday)


Plenary lecture 1P1 | Chair: Gabriella Endre
Signal perception and transduction
Myriam Charpentier
Calcium signalling in symbioses


Plenary lecture 1P2 | Chair: Gabriella Endre
Signal perception and transduction
Masayosi Kawaguchi
Regulatory System Evolution of Symbiotic Organ Development


Plenary lecture 2P1 | Chair: Gabriella Endre
Biochemistry of key processes and enzymes
Luis M. Rubio
Expression and maturation of nitrogenase components in mitochondria




Plenary lecture 3P1 | Chair: Éva Kondorosi
Infection and invasion
Jeremy Murray
Connecting the Dots: Gene Regulatory Networks in Rhizobial Infection


Plenary lecture 3P2 | Chair: Éva Kondorosi
Infection and invasion
Makoto Hayashi
Regulation of infection in root epidermis and cortex for nodulation


Plenary lecture 4P1 | Chair: Éva Kondorosi
Interplay of symbiotic interactions
Martin Parniske
A novel component of the CCaMK/CYCLOPS complex regulates root nodule symbiosis




Parallel section 1
Signal perception and transduction
Chairs:  Simona Radutoiu,
Pascal Gamas

Parallel section 2
Biochemistry of key processes and enzymes
Chairs:  Ray Dixon, Christian Staehelin

15 min talks

Florian Frugier
DELLA-mediated gibberellin signaling is a direct regulator of Nod Factor signaling and rhizobial infection

Yi-Ping Wang
Genetic requirements for biosynthesis and activity of FeFe nitrogenase

Dugald Reid
Identification of genes controlling cytokinin homeostasis during nodule development in Lotus japonicus

Nico Nouwen
The role of rhizobial (NifV) and plant (FEN1) homocitrate synthases in symbiotic nitrogen fixation

Xia Li
miRNAs regulate nodule number in soybean

Rui Maria Lima
The role of the signal peptide peptidase in nodule development and symbiosis

Katharina Markmann
A micro RNA acts as a signal in systemic control of nodulation symbiosis

José Jiménez-Zurdo
Sinorhizobium meliloti YbeY: a novel endoribonuclease involved in RNA-mediated gene silencing


Jean-Francois Arrighi
From the genetic map to the genome assembly of the Nod factor-independent Aeschynomene evenia to shed light on the evolution of nodulation

Kira Gysel
Comparative biochemical studies of Lotus japonicus LysM receptor like kinases

Anton Sulima
The pea (Pisum sativum L.) receptor-like kinase gene LykX, the most prominent candidate for Sym2, is required for successful penetration of rhizobia into the root hair

Carmen Sanchez-Canizares
Regulation of bacterial metabolism by the phosphotransferase system (PTSNtr)

Eiichi Murakami
Lotus-Rhizobium symbiosis is facilitated by the epidermal Nod factor receptor

Christian Staehelin
The Nod factor hydrolase of Medicago truncatula: an example of symbiosis-related neofunctionalization

Katja Katzer
Identification of a novel component of the CCaMK/CYCLOPS complex

Michael Göttfert
From symbiosis to biotechnology: The metal ion-inducible autocleavage (MIIA) domain

Carole Laffont
KNAT3/4/5-like KNOX transcription factors regulate symbiotic nodule organ development in Medicago truncatula potentially through the MtEFD/MtRR4 cytokinin-related regulatory module

Laure Decamps
Heterologous expression of enzymes of the nitrogenase pathway

Michael Djordjevic
Nodule and lateral root development are mediated by independent pathways downstream of the MtCEP1 peptide / CRA2 receptor in Medicago truncatula

Daniel Hsieh
Exploring the function of the inorganic phosphate transporter (PiT)-associated protein in Sinorhizobium meliloti




Parallel section 3
Infection and invasion
Chairs:  Thomas Ott, Krzysztof Szczyglowski

Parallel section 4
Interplay of nitrogen-fixing and mycorrhizal symbioses
Chairs:  Allan Downie, Caroline Gutjahr

15 min talks

Macarena Marin
Diversity of nodulation and infection in Lotus x Rhizobium combinations revealed dynamic evolutionary processes

Rene Geurts
Parasponia and Trema comparative genomics to provide insight in an evolutionary trajectory towards rhizobium symbiosis

Fernando Sorroche
Autoregulation of infection in the Sinorhizobium meliloti-Medicago symbiosis

Oksana Shtark
Arbuscular mycorrhiza development in pea mutants impaired in early nodulation genes including putative orthologs of NSP1 and NSP2

Maitrayee DasGupta
Role of SYMRK in synchronising the epidermal and cortical responses in root nodule symbiosis

Caroline Gutjahr
A CCaMK-CYCLOPS-DELLA complex regulated trancription in arbuscular mycorrhiza

David Chiasson
The unique brush allele reveals redundancy in a cluster of channel proteins during root development and infection by rhizobia

Juan Imperial
Differential Host-Selection Behaviour in the Rhizobium leguminosarum bv. viciae – Legume Symbiosis


Andreas Niebel
NF-Y TFs as key regulators of nodule development and infection.

Rosa Elena Andrade Aguirre
Do you want to join the complex? Towards the identification of new CCaMK/CYCLOPS interactors

Fang Xie
SCARN a novel class of SCAR protein that is required for root-hair infection during legume nodulation

Olga Kulaeva
The process of bacteroid differentiation in pea (Pisum sativum L.) is controlled by symbiotic genes that regulate the expression of the NCR gene family

Fernanda de Carvalho-Niebel
Host cell reprogramming for rhizobial root infection

Anna Zdyb
Expression of a rhizobial efflux system and its associated transcriptional regulator during nodule development

Yasuyuki Kawaharada
The ERF Required for Nodulation1 (ERN1) transcription factor is required for root nodule infection in Lotus japonicus

Marcela Mendoza-Suárez
Rhizobial competition: getting to the root of the problem

Marion Cerri
Regulation of Lotus japonicus ERN1 by the CCaMK/CYCLOPS complex constitutes a central step in the transcription factor network controlling bacterial accommodation

Anna Igolkina
Plant as an evolutionary driver of symbiotic microbiome

Annet Westhoek
Policing the gate: can pea plants stop rhizobial cheats from entering?

Kritarth Seth
Effect of phosphate solubilization on nitrogen fixation in clover

Thomas Ott
Molecular control of receptor mobility shifts during rhizobial infection

Katrin Petersen
Quorum sensing controls phenotypic heterogeneous expression of the autoinducer synthase gene traI via copy number control of pNGR234a in the plant symbiont S. fredii NGR234




27th August (Saturday)


Plenary lecture 5P1 | Chair: Pepe Palacios
Functioning of the N-fixing symbioses /bacteria/
Philip Poole
Metabolic transitions of rhizobia


Plenary lecture 5P2 | Chair: Pepe Palacios
Functioning of the N-fixing symbioses /bacteria/
Anke Becker
Plasticity of alpha-rhizobial genomes: a cell biological perspective


Plenary lecture 6P1 | Chair: Pepe Palacios
Biological nitrogen fixation in non-legume environments
Rachel Foster
Diatom-N2 fixing symbioses: making the most in a nutrient deplete open ocean




Plenary lecture 5P3 | Chair: Péter Kaló
Functioning of the N-fixing symbioses /plants/
Michael Udvardi
Deconstructing symbiosis: Loss-of-function mutations reveal key genes for symbiotic nitrogen fixation in Medicago truncatula


Plenary lecture 5P4 | Chair: Péter Kaló
Functioning of the N-fixing symbioses /plants/
Jean-Michel Ané
A band of misfits: role of unexpected proteins in the plant symbiotic signaling pathway


Plenary lecture 7P1 | Chair: Péter Kaló
Free-living nitrogen fixation
Enrique Flores
Intercellular communication in the diazotrophic filament of heterocyst-forming cyanobacteria




Parallel session 5A
Functioning of the nitrogen-fixing symbioses /bacteria/
Chairs:  Sharon Long, Emanuele Biondi

Parallel session 6
Biological nitrogen fixation in non-legume environments
Chairs:  Barbara Reinhold-Hurek, Adriana Hemerly

15 min talks

Allan Downie
A hypothesis for the acquisition and evolution of peptides controlling differentiation of nitrogen fixing rhizobia in legume nodules

Adriana Hemerly
Endophytic diazotrophic bacteria: the plant understanding of this beneficial association

Emanuele Biondi
Bacterial cell cycle and bacteroid differentiation are linked in Sinorhizobium meliloti

Barbara Reinhold-Hurek
A glance at the endophytic lifestyle of Azoarcus sp. BH72: Factors contributing to endophytic competence

George diCenzo
Development of a permissive platform for identification of the minimal rhizobial symbiotic genome and forward genetic analyses

Barney Geddes
Discovery of a novel rhizopine synthesis pathway paves the way for synthetic symbioses and nitrogen fixing cereal crops

Etelka Kovacs
Characterization of Sinorhizobium meliloti mutants with increased resistance towards NCR peptides


Lightning talks

Marta Robledo
sRNA-mediated regulation of the cell cycle master regulator CtrA in Sinorhizobium meliloti

Yongliang Yan
The RNA Chaperone Hfq is a global regulator in the nitrogen-fixing Pseudomonas stutzeri A1501


Ken-ichi Yoshida
Inactivation of PhaR involved in poly-beta-hydroxybutyrate accumulation in Bradyrhizobium japonicum USDA110 and its pleiotropic effects

Vijay Singh
Identification and functional characterization of genes involved in carbon source utilization in A. brasilense Sp7

Rachel Wheatley
Insertion Sequencing in Rhizobium leguminosarum bv. viciae 3841

Luciana Fernandes de Brito
Development of tools for transformation and gene expression in Paenibacillus species and complete genome sequence of Paenibacillus riograndensis SBR5


Chang Fu Tian
MucR is required for transcriptional activation of conserved ion transporters to support nitrogen fixation of Sinorhizobium fredii in soybean nodules

Christopher Waite
The regulation of nitrogen fixation and assimilation in the associative diazotroph Klebsiella oxytoca M5a1


Kathrin Wippel
Stringent response-mediated transcriptional changes in the Medicago-Sinorhizobium root nodule symbiosis

Paramasivan Ponraj
Engineering a biased plant rhizosphere to establish synthetic symbioses in cereals


Hassen Gherbi
Signaling pathway in the actinorhizal root nodule symbiosis



Denis Warshan
Functional genomics of cyanobacteria in symbiosis with boreal feather mosses



Nguyen Van
Evolution of the actinorhizal symbiosis: analysis of bacterial genomes of the basal cluster




Parallel session 5B
Functioning of the nitrogen-fixing symbioses /plants/
Chairs:  Katharina Pawlowski, Jean-Michel Ané

Parallel session 7
Free-living nitrogen fixation
Chairs: Anton Hartmann, Rachel Foster

15 min talks

Pascal Gamas
Epigenetic regulation is essential for the development of indeterminate nodules

Jorg Schumacher
Synthetic rebalancing of nitrogen fixation and nitrogen assimilation in diazotrophs.

Marianna Nagymihály
Dynamic changes in chromatin structure during endoreduplication regulate expression of nodule-specific NCR genes in Medicago truncatula

San-feng Chen
Using synthetic biology to increase nitrogenase activity

Pascal Ratet
Medicago truncatula nodule-root (noot) genes are guards of the symbiotic organ identity

Corinne Appia-Ayme
Regulation of alternative nitrogenase expression by σ54-dependent activator homologs in Azotobacter vinelandii

Joachim Schulze
Legume shoots induce a 24 h nitrogenase-activity rhythm under the influence of various environmental cues by a common molecular mechanism

Keisuke Inomura
Macro-molecular model indicates multiple oxygen management strategies by Crocosphaera watsonii

Lightning talks

Gabriella Endre
The role of U-box ubiquitin ligases during plant-microbe interactions

Sofie Vonlanthen
Isolation and characterization of two new nitrogen fixing unicellular cyanobacteria from the Indian Ocean

Manuel Gonzalez-Guerrero
MtNramp1, MtZIP6, and MtCOPT1 are respectively responsible for iron, zinc, and copper uptake by Medicago truncatula nodule cells.

Mónica Navarro Rodríguez
Molybdenum metabolism in Azotobacter vinelandii

Eric Boncompagni
Thioredoxin 1 s1 is essential for bacterial terminal differentiation in the nitrogen-fixing symbiosis in M. truncatula

Agneta Norén
Studies of DraB, a small thioredoxin like protein in Rhodospirillum rubrum with an unknown function encoded within the dra operon

Stig Andersen
Genetic dissection of nodulation signalling using the LORE1 insertion mutant collection


Jesus Montiel Gonzalez
The profile of NCR peptides produced by the legume host correlates with the morphotype of the bacteroids


Szilard Kovacs
Identification of novel symbiotic plant genes with the help of M. truncatula Tnt1 insertional mutants


Katharina Schiessl
What defines and regulates nodule identity and organogenesis?





28th August (Sunday)


Plenary lecture 8P1 | Chair: Attila Kereszt
On the interface of symbiotic/pathogenic interactions
Gary Stacey
Role of plant innate immunity in the legume, nitrogen fixing symbiosis


Plenary lecture 8P2 | Chair: Attila Kereszt
On the interface of symbiotic/pathogenic interactions
Peter Kaló
The Medicago truncatula NAD1 gene is essential for the persistence of bacteroids in symbiotic nodules


Plenary lecture 9P1 | Chair: Attila Kereszt
Evolution, diversity and ecology
Pierre-Marc Delaux
Evolution of symbioses: from phylogeny to intelligent design




Parallel session 8
On the interface of symbiotic/pathogenic interactions
Chairs:  Rene Geurts, Pascal Ratet

Parallel session 9
Evolution, diversity and ecology
Chairs:  Euan James, Peter Young

15 min talks

Shin Okazaki
Symbiotic roles of the type III secretion system in Bradyrhizobium elkanii

Arjan van Zeijl
Genetic dissection of the rhizobium nodulation trait using interspecific crosses between symbiotic Parasponia and non-symbiotic Trema species

Benjamin Gourion
Exploring the immune status of nodules

Ruben Garrido Oter
Root nodule symbiosis in Lotus japonicus drives the establishment of distinctive rhizosphere, root, and nodule bacterial communities

Péter Körmöczi
The incompatible interaction between Medicago truncatula A17 and Sinorhizobium meliloti RM41 induces early nodule senesce

Vladimir Zhukov
Molecular evolution of paralogous symbiotic receptor kinase genes in pea (Pisum sativum L.)

Sebastian Schornack
The Medicago api gene is required for full colonisation by P. palmivora as well as nitrogen fixing bacteria


Lightning talks

Zoltán Bozsóki
Symbiosis or defense: the molecular mechanism involving LysM receptors of the model legume Lotus japonicus

Claude Bruand
Stress-induced DNA double-strand break NHEJ repair in Sinorhizobium meliloti: a function in lateral gene transfer?

Jongho Sun
Assessing the relevance of a range of polysaccharide signaling molecules for activation of symbiotic signaling

Alice Checcucci
Mixed nodules in Sinorhizobium meliloti – Medicago sativa symbiosis suggest the presence of a cheating behavior

Irina Leppyanen
The investigation of the mechanisms by which pea plants discriminate and respond to structurally related COs signals from symbiotic and pathogenic fungi

Elizaveta Chirak
Structure and functional design of the plasmid regions harboring sym genes in Rhizobium leguminosarum: new evidence for intensification of horizontal gene transfer and narrowing the host range in rhizobia evolution

Nicolas Busset
Hopanoids play an important role in Bradyrhizobium strains during their free-living and symbiotic states

Mitchell Andrews
The range of rhizobia in New Zealand soils

Francisco López-Baena
Unraveling plant cellular targets for the Rhizobium-specific effectors NopL and NopP

Delphine Capela
Genetic evidence that local legume sanctions drive the emergence of symbiotic nitrogen fixation

Dong Wang
Specialised protein secretion in plant-microbe symbioses

Anastasiia Kimeklis
Symbiotic divergence of Rhizobium leguminosarum strains from relict legume Vavilovia formosa: a background for identification of novel biovar

Getinet Desalegn
Rhizobia inoculation reduces Didymella pinodes impacts on photosynthetic efficiency of Pisum sativum

Sara Moeskjaer
The impact of host genotype and geographical origin on Rhizobium leguminosarum genetic diversity


Vladimir Kopat
Evolution of fixNOQP genes encoding for the high-affinity cytochromoxidase: insight from the genomes of symbionts from the relic legume Vavilovia formosa



Kathryn Wigley
Carbon utilisation by strains of Rhizobium spp. in sterile soil





Plenary lecture 10P1 | Chair: Jens Stougaard
Commonalities and specialities of symbiotic interactions
Peter Mergaert
Widespread use of antimicrobial peptides in bacterial symbiosis


Plenary lecture 10P2 | Chair: Jens Stougaard
Commonalities and specialities of symbiotic interactions
Martin Grube
The diverse bacterial side of lichens: Key to a new concept of symbioses


Plenary lecture 11P1 | Chair: Jens Stougaard
The present and the future agricultural use of BNF
Giles Oldroyd
Dissecting and Engineering Symbiosis Signalling


Plenary lecture 11P2 | Chair: Jens Stougaard
The present and the future agricultural use of BNF
Ken Giller
The broader benefits of N2-fixation